BIO270 Vertebrate Zoology

Fall 2018

Laboratory guide

 

 

 









Lab Schedule

 

 

Laboratory Schedule: Note:  This schedule may be subject to change as the course proceeds

 

Date(s)         Topics                                                                                          Exercises         

Aug 14

Lab Preparation - Dissection and Osteological Mounts  

Aug 21

Vertebrate Phylogeny I: Simple Chordates to Agnathans KZ 1, 2

Aug 28

Vertebrate Phylogeny II: Gnathostomes; Tissue Types KZ 2, 3

Sept 4

 

Vertebrate Development and Design: Development; Allometry; Bone Metrics; Joint Mechanics

Handouts

 

Sept 11

LAB EXAM I  

Sept 18

 

Skull and Dentition; Taxonomic Keys

 

KZ 5; Handouts

Sept 25

 

External Anatomy; Integument

Taxidermy I: Study Skins, Carcass Skinning and Debridement

KZ 4, 6

 

Oct 2

 

Axial Skeleton & Muscles

Taxidermy II: Skeletal Reconstruction

KZ 5, 6

 

Oct 9

no lab - Fall Break  

Oct 16

LAB EXAM II  

Oct 23

Appendicular Skeleton & Muscles

Taxidermy III: Skeletal Mounting

KZ 5, 6

Oct 30

Body Cavities; Digestive System; Respiratory System KZ 7, 8

Nov 6

Circulatory System; Lymphatic System; Blood KZ 8

Nov 13

LAB EXAM III  

Nov 20

No Lab - Thanksgiving Break  

Nov 22

Excretory System; Reproductive Systems; Endocrine System KZ 9; CR 14

Dec 4

Nervous System; Special Senses KZ 10; CR 13

TBA

LABORATORY AND CLASS FINAL EXAMS  

 

                                                                            

KZ = Kardong, K.V. & Zalisko, E.J. (2014) Comparative Vertebrate Anatomy.(7th Ed.) 

McGraw Hill.  Dubuque, Iowa.

 

CR = Chaisson, R.B. & Radke, W.J.  (1993)  Laboratory Anatomy of  the Vertebrates.  W. C. Brown.  Dubuque, Iowa.  (We will be using this as a supplemental guide to the endocrine and neural sensory systems, which are not covered in the Kardong & Zalisko Guide.)

                                                                          

 

back to top=


CARE AND USE OF MICROSCOPES AND SLIDES

 

The microscopes that you will be using for this course are binocular compound scopes.  You may use these at any time, as long as you do not disrupt or interfere with other ongoing laboratory or class sessions.  Demonstration slides will be set up on some of these scopes and should remain on the work tables in the back o f the room.  You should familiarize yourself as soon as possible with how the microscopes work.  Here are a few rules for use and care to keep in mind while you use the scopes:

 

-     If you have never used a compound binocular microscope before, ask the instructor to demonstrate it before you use it.  Microscope use will be demonstrated at the start of the course, so pay close attention during this demonstration.

 

-     Always carry the microscope with one hand grasping the neck and the other supporting the base.  Make sure that the cord is securely wrapped around the base.  Never try to support a microscope by the oculars (eyepieces) or the stage.  Never carry a microscope with a slide on the stage.

 

-     When you are finished with the microscope, put it away where you found it.  Remove the slide, turn off the light, unplug it, wrap the cord around the base, put the cover on, place it gently in its cabinet, and close the door.

 

-     The microscope has four objectives.  The highest power objective is an oil immersion lens.  This means that it will only be in focus and provide a clear image when there is a droplet of oil between it and the slide.  In general, the only time you will need to use oil immersion will be on some demonstration slides which will be set up for you.  If you feel that you need to use oil immersion on your own with the loan slide collections, consult with the instructor first.  With oil immersion the objective is very close to the slide, so use extreme caution (see next point).

 

-     When viewing a slide always start at low power.  Start with the microscope focused down as far as possible, insert the slide, and bring the slide into focus by focusing up.  Use the coarse focus knob first, then the fine focus. Never focus down on the coarse focus while looking through the microscope.   The microscope is parfocal (more or less), which means that if the slide is in focus at low power, it will remain approximately in focus as you switch to higher powers.  Use only the fine focus at higher powers.  Use the coarse focus only with the lowest power objective.  The reason for all of these rules will be obvious when you view your first slide.  It is quite possible to run the higher power objectives into the slide, thereby breaking the slide (bad) or scratching the objective (very bad).

 

-     Lens papers, lens cleaning solution, and KimWipes will be provided at the front desk. Never use any other materials or solutions to clean microscope lenses or slides.

 

-     Use the stage control knobs for moving the slide.  Don't push directly on the slide or the stage.

 

-     If you are using a microscope that doesn't seem to work properly, tell the instructor or leave a note on the microscope so that it can be repaired as soon as possible.

 

-     Handle the loan collection slides with care.  Keep them in their box slots when not in use, not piled on the table, or on your books, or in your pockets.  Handle slides only over the tables, not over the floor.  Make sure that the slide box is latched before you carry it anywhere.  Make sure that the box is right side up before you open it.  If you do break a slide, tell the instructor so that it can be replaced as soon as possible.

 

The binocular microscope is designed to be looked through binocularly, that is with both eyes.  If the two images don't seem to line up, try adjusting the interocular distance by sliding the two oculars at their base.  The light intensity may be adjusted by turning the rheostat knob.  In general you will want it near the maximal value.  The condenser should be adjusted (by moving it up or down) to maximize the apparent brightness and provide uniform illumination to the field.

 

The adjustment of the diaphragm will depend on the lighting effect that you want.  A good general purpose setting will be to close the diaphragm just enough to get some dimming at the rim of the field.   If you want greater contrast (at the expense of some resolution), close the diaphragm more.  This will be especially useful when viewing thin, transparent specimens such as blood smears, areolar C.T., etc. or specimens of varying optical density such as bone. If you need maximal resolution and illumination, open the diaphragm fully.  This will be useful for thick, optically uniform specimens.

 

back to top=


CARE AND USE OF BONES AND SKELETONS

 

Living bones are remarkably sturdy structures.  Dead bones may be surprisingly fragile.  The skeletons we will be using for this class do not have movable joints, so do not try to bend them.  For obvious reasons, never use pencils, pens, or markers as pointers.  A good rule of thumb for handling the skeletons and disarticulated bones, especially the skull, feet, and hands, is to treat them as if they were irreplaceable.  They are. 

       

back to top=


CARE AND USE OF PRESERVED SPECIMENS

 

In this course you may have the opportunity to dissect and handle preserved animals and animal organs.  These guidelines also apply to preserved specimens.

 

-     Always wear gloves when handling animal tissue.  Avoid touching your face with the gloves.  Always remove and dispose of the gloves, then wash your hands when you are done.

 

-     If you feel faint, first tell someone, then find a quiet place and sit down.  If you still feel faint, get someone to help you lie down on one of the tables or help you leave the room to get some fresh air.  Don't be shy or embarrassed about admitting that you feel badly and asking for help.  In a similar vein, keep an eye on those around you, and be ready to help them if they appear to need it. 

 

-     Preserved specimens must not be allowed to dry out.  A single two-hour exposure will ruin most organ preps.  Whenever you are finished examining a specimen, immediately return it to its fluid-filled container, and seal the container.

     

-     Handle the specimens with extreme care.  Do not pull on structures to see behind them.  Do not directly handle blood vessels or nerves; use the probes to gently lift them or displace them to one side.  Remember that you or someone else put quite a bit of time into dissecting the specimen, that once you damage something it will stay damaged, and that material will be used on the exams whether it is damaged or not..

 

-     Use only probes or your fingers as pointers.  Point, don't poke.  Never use pens or pencils as pointers.

 

back to top=