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September 11, 2010

Experimental Design and Analysis with Jewel Wasps

This page: http://pierce.wesleyancollege.edu/faculty/MSP/sept2010.html

 

Organizer:

 

Barry K. Rhoades, Ph.D.   Professor of Biology

 

Schedule:

Support

Materials

Jewel wasp biology

      jewel wasp background (PPT)

Jewel wasp laboratory exercises - BIO 315 Animal Behavior

      behavioral sampling

      reproductive sex ratios

Jewel wasp sample results

      BIO315 result matrices and chi-square analysis

Jewel wasp supplies

      jewel wasp cultures

      blowfly (host) pupae

      culture tubes

      petri dishes

      carbon dioxide anesthetizer

8:30 - 9:00

 

 

Experimental Design - idealized sequence

   observation

   background research

   question

   hypothesis

   operationalization

   experimental test

      null hypothesis

      alternate hypothesis

   data collection

      categorical

      quantitative

   data analysis

   conclusions

   dissemination/presentation

Experimental Design - practical aspects

   what is my budget?

   how much time do I have?

   what do I need to buy?  

   what can I make more cheaply?

   how much data do I need?

   what is the most appropriate analysis?

   what is the most appropriate presentation format?

   

9:00 - 9:30

 

 

Jewel Wasp Reproductive Sex Ratios

   background and experimental question

      jewel wasp life cycle

      haplodiploid sex determination

      Hamiltonian sex ratio considerations

   possible experimental questions/hypotheses and designs

      foundress order

      foundress competition level

      foundress virginity

 

9:30 - 9:35

BREAK

 

9:35 - 10:00

Jewel Wasp Care and Culture

   ordering supplies

   jewel wasp cultures - do's and don'ts

   Sarcophaga pupae - do's and don'ts

   distinguishing male from female wasps

   eye color variants

   collecting virgin wasps vs. mated females

   anesthetizing, handling, and transferring wasps

   keeping wasps alive for more than three days

 

10:00 - 10:30

 

 

Your Experiment

   the experimental design

      storing pupae - must keep refrigerated!

      separating eclosed females

      setting up oviposition chambers

      transferring oviposited host pupae to culture tubes

      sexing and counting offspring

Sample Data Analysis

      tabulating data

      comparing and graphing sex rations

      a Chi-square statistical test using Excel

 

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